Figure 1.

The sequence of nc886 and its natural expression, in comparison to ectopic expression methods. The three sequences on the top are from miRBase (red letters: miR-886-5p and -3p), from the NCBI database, and experimental determination. Pol III promoter and termination elements are highlighted in green shadow. The nc886 RNA is drawn in wavy lines in which red portions designate miR-886-5p and -3p. Improper ectopic expression of nc886 (and its alleged products miR-886-5p and -3p) is illustrated in faint orange boxes. Magenta blunt arrows with a question mark indicate and potential side effects of illegitimate expression. See the main text for details.