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. 2022 Apr 3;10(4):840. doi: 10.3390/biomedicines10040840

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Expression Pattern of CD44v6 in Laser-induced Choroidal Neovascularization (CNV). (A) CD44v6-expression in proliferating endothelial cells in a mouse model of laser-induced CNV. (B) Absence of CD44v6 in quiescent retinal blood vessels. Scale bar represents 100 µm. Primary antibodies were monoclonal rat anti-CD44v6 (clone 94A) and polyclonal rabbit anti-Collagen IV, visualized by secondary antibodies chicken anti-rabbit Alexa Fluor^TM 647 and donkey anti-rabbit Alexa Fluor^TM 488. Whole-mount staining was performed as described previously [8].