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. 2022 Apr 8;12(8):972. doi: 10.3390/ani12080972

Table 2.

Brush-directed behaviors performed by group-housed heifers (7 to 8 heifers/group) for each 6 h observation period, regardless of brush quantity treatment (2 vs. 4 brushes/pen, n = 4 groups/treatment).

Period 1
Behavior 1 2 3 4 SE 2 F-Value 3 p-Value
Competition events (no.) 8.6 a 4.9 bc 3.1 b 7.1 ac 1.08 6.9 (3,16.4) 0.003
Bout Frequency (no.) 4 18.8 a 17.1 ab 12.2 b 15.8 ab 14.50 5.2 (1,16.3) 0.011
Bout Duration (s/bout) 4 22.5 b 19.9 b 41.2 a 41.6 a 2.60 20.2 (1,16.7) <0.001

1 Behavior observations were performed across four 6 h periods, defined as periods 1–4 (0–6, 18–24, 120–126, and 138–144 h after exposure, respectively). 2 Pooled standard error. 3 Degrees of freedom associated with each F statistic are reported in parentheses. 4 Bout characteristics based on 2 pooled inter-bout criteria calculated from 0–6 and 18–24 h of exposure (periods 1 and 2, when the brushes were most novel) vs. 120–126 and 138–144 h of exposure (periods 3 and 4). a–c Superscripts that differ within row indicate significant differences (p < 0.05).