Fig. 4.

miR-15b-5p facilitates the proliferation and invasion of GC cells by targeting BRSM1. A Expression of BRMS1 determined by RT-qPCR in AGS and MKN-45 cells overexpressing BRSM1. AGS and MKN-45 cells were transfected with miR-15b-5p mimic or combined with BRMS1. B Western blot analysis of BRMS1, E-cadherin, N-cadherin and Vimentin proteins in cells. C Colony formation of AGS and MKN-45 cells. D Invasion of AGS and MKN-45 cells measured by Transwell assay. E Matrix adhesion ability of AGS and MKN-45 cells. Each experiment was conducted three times independently. * p < 0.05, compared with AGS and MKN-45 cells transfected with Vector or mimic-NC + Vector. # p < 0.05, compared with AGS and MKN-45 cells transfected with miR-15b-5p mimic + Vector