Fig. 7.

M2 macrophage-derived EVs induce the metastasis of GC via DAPK1 impairment in vivo. A The invasion of AGS and MKN-4 cells treated with EV-miR-15b-5p mimic and EV-mimic-NC determined with Transwell assay (scale bar = 50 μm). B Western blot analysis of BRMS1 and DAPK1 proteins in cells treated with EV-mimic-NC + Vector, EV-miR-15b-5p mimic + Vector or EV-miR-15b-5p mimic + BRMS1. C Western blot analysis of BRMS1, DAPK1, E-cadherin, N-cadherin, and Vimentin proteins in cells treated with M2-EV + Vector and M2-EV + DAPK1. D Colony formation of AGS and MKN-45 cells treated with M2-EV + Vector and M2-EV + DAPK1. E Invasion of AGS and MKN-45 cells treated with M2-EV + Vector and M2-EV + DAPK1 measured by Transwell assay. F Matrix adhesion ability of AGS and MKN-45 cells treated with M2-EV + Vector and M2-EV + DAPK1. G HE staining analysis of the lung tissues of mice treated with M2-EV + Vector and M2-EV + DAPK1 (scale bar = 50 μm). H Survival curve of nude mice treated with M2-EV + Vector and M2-EV + DAPK1. Each experiment was conducted three times independently. n = 8 for mice following each treatment. * p < 0.05, compared with AGS and MKN-45 cells or mice treated with PBS + Vector. # p < 0.05, compared with AGS and MKN-45 cells treated with EV-mimic NC + Vector, or AGS and MKN-45 cells or mice treated with M2-EV + Vector. ^ p < 0.05, compared with AGS and MKN-45 cells treated with EV-miR-15b-5p mimic + Vector