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. 2022 Mar 23;11(4):391. doi: 10.3390/pathogens11040391

Table 1.

Concentration of 2,4-diacetylphloroglucinol (2,4-DAPG), pyoluteorin (PLT), and pyrrolnitrin (PRN) in monocultures of P. protegens (strain DSMZ 13134). Monocultures were grown in Luria–Bertani, King B, and a modified King B broth at 4 °C, 25 °C, and 30 °C and incubating them for 24 h and 7 days.

Monocultures of P. protegens (Strain DSMZ 13134) Mean Concentration ± SD 1 (mg/L)
Culture Medium Incubation Temperature (°C) Incubation Period 2,4-DAPG PLT PRN
Luria–Bertani broth 4 24 h 0.17 ± 0.00 0.01 ± 0.00 <LOD 2
7 days <LOD 3.63 ± 0.38 <LOD
25 24 h <LOD 3.77 ± 0.30 <LOD
7 days <LOD 0.66 ± 0.00 <LOD
30 24 h 0.26 ± 0.00 0.81 ± 0.38 <LOD
7 days <LOD 0.35 ± 0.24 <LOD
King B broth 4 24 h <LOQ 3 0.32 ± 0.00 <LOD
7 days <LOQ 8.03 ± 0.00 <LOD
25 24 h <LOQ 6.12 ± 0.42 <LOD
7 days <LOQ 2.34 ± 0.01 <LOD
30 24 h <LOQ 0.37 ± 0.20 <LOD
7 days <LOQ 0.49 ± 0.50 <LOD
Modified King B broth 4 24 h <LOD 0.27 ± 0.00 <LOD
7 days <LOD <LOD <LOD
25 24 h <LOD <LOD <LOD
7 days <LOD 1.10 ± 0.02 <LOD
30 24 h <LOD 4.52 ± 0.49 <LOD
7 days <LOD 10.21 ± 3.18 <LOD

1 The values represent the mean and standard error of the mean for three replicates, each represents one monoculture of P. protegens (strain DSMZ 13134). Values were rounded to two significant digits. 2 <LOD means under the limit of detection, being 0.1 mg/L for every compound. 3 <LOQ means under the limit of quantification, being approximately 0.5 mg/L for each compound.