Table 1.
Primer sequences used for the amplification of fragments from genes encoding murine PrP (PRNP), murine Nrf2 (NFE2L2), murine heme oxygenase 1 (Hmox), murine c-glutamyl-cysteine-ligase (Gclm), and ACTB (β-Actin, reference gene).
| Primer | Gene | Sequence (5′-3′) | Amplified Fragment (bp) |
|---|---|---|---|
| qRT_mo_HMOX1F | HMOX1 | CAG TCG CCT CCA GAG TTT CC | 284 |
| qRT_mo_HMOX1R | TAC AAG GAA GCC ATC ACC AGC | ||
| qRT_mo_GCL-MF | GCLM | CTG CAA AAC TGT TCA TTG TAG G | 280 |
| qRT_mo_GCL-MR | CTA TTG GGT TTT ACC TGT G | ||
| qRT_mo_NFE2L2F | NFE2L2 | GCA ACT CCA GAA GGA ACA GG | 142 |
| qRT_mo_NFE2L2R | GTG GGC AAC CTG GGA GTA G | ||
| moPrP_103F | PRNP | AAT CAG TGG AAC AAG CCC AG | 103 |
| moPrP_103R | CCA GCA TGT AGC CAC CAA G | ||
| mo_actin_Alt-F | ACTB | CAG CTT CTT TGC AGC TCC TT | 375 |
| mo_actin_Alt-R | CAC GAT GGA GGG GAA TAC AG |