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. 2022 Mar 28;10(4):788. doi: 10.3390/biomedicines10040788

Figure 5.

Figure 5

Manipulation of circ0087207 modulated apoptosis but not intracellular ROS levels in LHON patient iPSC-derived RGCs. (A) Map of the plasmid pcDNA3.1(+) ZKSCAN1 MCS hsa_circ_0087207 for circRNA_0087207 overexpression; (B) validating the overexpression of hsa_circ_0087207 using quantitative real-time PCR. (C) Flow cytometry and (D) its quantification showed the intracellular ROS accumulation in normal iPSC-derived RGCs (upper) and carrier iPSC-derived RGCs (lower) after hsa_circ_0087207 overexpression. (E) Flow cytometry and (F) its quantification showed the apoptosis rate in normal iPSC-derived RGCs (upper) and carrier iPSC-derived RGCs (lower) after hsa_circ_0087207 overexpression. (G) Map of the plasmid plko1 puro-shRNA4 hsa_circ_0087207 used for circ0087207 knockdown; (H) validating the knockdown of hsa_circ_0087207 using quantitative real-time PCR. (I) Flow cytometry and (J) its quantification showed the intracellular ROS accumulation in LHON patient iPSC-derived RGCs after hsa_circ_0087207 knockdown. (K) Flow cytometry and (L) its quantification showed the apoptosis rate of LHON patient iPSC-derived RGCs after hsa_circ_0087207 knockdown. * p < 0.05; ** p < 0.01. ns p > 0.05.