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. 2022 Apr 15;23(8):4405. doi: 10.3390/ijms23084405

Figure 2.

Figure 2

Docosahexaenoic acid (DHA) suppressed tert-butyl hydroperoxide (tBHP)-induced intracellular reactive oxygen species (ROS) in immortalized Fischer rat Schwann cells 1 (IFRS1). (a) Treatment with tBHP for 30 min induced ROS production in IFRS1 cells as monitored by fluorescence microscopy whereas pretreatment with 10 μM DHA for 12 h significantly suppressed tBHP-induced ROS production. Scale bar: 100 μM. (b) Quantification of fluorescent intensity of ROS in IFRS1 cells. Each value represents the mean ± S.E. of eight experiments. **: p < 0.01 compared with the control; ##: p < 0.01 compared with the 50 μM tBHP control.