Figure 4.

Docosahexaenoic acid (DHA) suppresses tert-butyl hydroperoxide (tBHP)-induced autophagy in immortalized Fischer rat Schwann cells 1 (IFRS1). (a–f) IFRS1 cells treated with 50 μM tBHP for 3 h in the presence or absence of pretreated 10 μM DHA were analyzed by Western blotting. Each value represents the mean ± S.E. of three experiments. *: p < 0.05 and **: p < 0.01 compared with the control; ^#: p < 0.05 and ^##: p < 0.01 compared with the 50 μM tBHP control. LC3: microtubule-associated protein 1 light chain 3; AMPK: AMP-activated protein kinase; ULK1: UNC51-like kinase.