Figure 8.

RAW 264.7 (4.0 × 10⁵/well) were seeded in six wells and incubated with 10 μg/mL of FP or P in the presence or absence of 100 U/mL of IFN-γ for 24 h. (A) iNOS and (B) TNFα genes expression was assessed using qRT-PCR analysis. Differences in gene expression were calculated using the 2^−∆∆Ct formula and RPL13A as reference gene. Results are expressed as mean ± SD of at least three experiments. * p <0.05. significantly different from the ctrl. Ctrl (control, untreated cells); FP (cells treated with fermented Pushgay); P (cells treated with Pushgay); IFN-γ (cells treated with IFNγ).