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. 2022 Apr 7;11(8):1256. doi: 10.3390/cells11081256

Figure 2.

Figure 2

Multiplex immunoassay indicates the elevation of plasma CCL1 and CCL27, and multiple cytokines in preeclamptic patients: plasma samples (n = 3, per group) from non-pregnant (NP), healthy pregnant or pregnant controls (PC), gestational hypertensive (GH) and preeclamptic (PE) participants were analyzed for the indicated cytokine/chemokine as biomarkers of systemic inflammation. The multiplex immunoassay was performed with the Millipore MILLIPLEX Cytokine Array Assay Kit, and the results were analyzed with a Bio-Plex 200. Sample analysis was performed in duplicates for each participant, using the Bio-Plex Multiplex System, powered by Luminex xMAP technology. Data were then analyzed using GraphPad Prism 9 and were summarized in box-and-whisker plots for each biomarker. The plots show minimum to maximum values and dots/points represent all raw data (replicate experiments inclusive). The medians and interquartile ranges of data are represented by the horizontal line through the box and width of the box, respectively. Statistical significance was determined by 1-way ANOVA and Bonferroni adjusted post hoc independent samples t-tests. p < 0.05 (*) or p < 0.01 (**) were considered significant.