Table 1.
Sequencing statistics and GenBank IDs for two HSV-1 genomes from NASA astronaut.
| Virus | GenBank ID | Input DNA ^
HSV Copy # |
Enriched Library ^
HSV Copy # |
Total # Reads * |
% HSV | # Reads Used for Assembly * | Average Coverage |
|---|---|---|---|---|---|---|---|
| In-flight rash (FD82) | ON152715 | 1.7 × 103 | 5.7 × 105 | 7.8 million | 80% | 6.2 million | 11,166X |
| Post-flight (R + 0 passive) saliva | ON152716 | 2.0 × 107 | 2.5 × 107 | 8.6 million | 79% | 6.8 million | 11,828X |
^ Values determined by qPCR on each sample after DNA isolation (see Methods for details). * All read counts refer to the number of forward reads only. “Total # reads” includes all forward reads, including unpaired and host-aligning reads. “# reads used for assembly” includes quality-trimmed, properly paired, HSV-specific forward reads.