Figure 3.

Effects of time at which lamellarin α 20-sulfate was added on inhibitory effects. The 293T cells were treated with lamellarin α 20-sulfate (150 µM) at the time points indicated in the upper panel, as indicated in the Materials and Methods section. The luciferase activities of DMSO-treated cells were always set to 1, and the relative values to the luciferase activities of the DMSO-treated cells are indicated. This experiment was repeated three times. Error bars indicate SD. When the differences between indicated groups are significant, the p values of Student’s t-test are indicated.