Table 2.
Probe | Sequence | Target rRNA | Specificity | FA ** |
---|---|---|---|---|
[%] | ||||
EUB338 | 5’-GCT GCC TCC CGT AGG AGT-3’ | 16S | Most bacteria | 35 |
EUB338II | 5’-GCA GCC ACC CGT AGG TGT-3’ | 16S | Planctomycetales | 35 |
EUB338III | 5’-GCT GCC ACC CGT AGG TGT-3’ | 16S | Verrucomicrobiales | 35 |
NON338 | 5’-ACT CCT ACG GGA GGC AGC-3’ | 16S | Control probe complementary to EUB338 | 35 |
ALF968 | 5’-GGT AAG GTT CTG CGC GTT-3’ | 16S | Alphaproteobacteria, except of Rickettsiales | 20 |
BET42a | 5’-GCC TTC CCA CTT CGT TT-3’ | 23S | Betaproteobacteria | 35 |
5’-GCC TTC CCA CAT CGT TT-3’ * | ||||
GAM42a | 5’-GCC TTC CCA CAT CGT TT-3’ | 23S | Gammaproteobacteria | 35 |
5’-GCC TTC CCA CTT CGT TT-3’ * | ||||
DELTA495a | 5’-AGT TAG CCG GTG CTT CCT-3’ | 16S | Most Deltaproteobacteria | 35 |
5’-AGT TAG CCG GTG CTT CTT-3’ * | and most Gemmatimonadetes | |||
DELTA495b | 5’-AGT TAG CCG GCG CTT CCT-3’ | 16S | Some Deltaproteobacteria | 35 |
5’-AGT TAG CCG GCG CTT CKT-3’ * | ||||
DELTA495c | 5’-AAT TAG CCG GTG CTT CCT-3’ | 16S | Some Deltaproteobacteria | 35 |
5’-AAT TAG CCG GTG CTT CTT-3’ * | ||||
CF319a | 5’-TGG TCC GTG TCT CAG TAC-3’ | 16S | Most Flavobacteria, some Bacteroidetes, some Sphingobacteria | 35 |
HGC69a | 5’-TAT AGT TAC CAC CGC CGT-3’ | 23S | Actinobacteria (high G+C Gram-positive bacteria) | 25 |
5’-TAT AGT TAC GGC CGC CGT-3’ * |
* Unlabelled competitor ** Formamide FA [%]: formamide concentration in the hybridisation buffer to ensure specific detection of target organisms.