Figure 2.

NaHCO₃ dose response impact on Bocillin-FL binding to purified PBP2a variant 246G; 50 µg/mL PBP2a was incubated with 50 µM Bocillin-FL and either 0, 6.25, 12.5, 25, 44, or 50 mM NaHCO₃. Relative Bocillin intensity was normalized to a control lane consisting of 25 µM Bocillin-FL incubated with 50 µg/mL 246G PBP2a run on each gel. Final data was normalized to the intensity of 50 µg/mL PBP2a incubated with 50 µM Bocillin-FL and no NaHCO₃ (with this value set equal to 1.0). Asterisks represent significant differences in Bocillin-FL intensity at the indicated NaHCO₃ concentration as compared to no NaHCO₃ (Student’s t-test, * p < 0.05, ** p < 0.01).