Figure 3.

Kinetic binding of Bocillin-FL to purified PBP2a variants 246G and 246E. (A) Kinetic Bocillin-FL binding without NaHCO₃. (B) Kinetic Bocillin-FL binding with NaHCO₃. A total of 50 µg/mL PBP2a was used in each Bocillin binding reaction. Relative Bocillin intensity was determined by normalizing absolute intensity values for each sample to the intensity of 25 µM Bocillin bound to 50 µg/mL 246G PBP2a in the absence of NaHCO₃ incubated for 20 min run on the same gel.