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. 2022 Apr 15;12(8):1362. doi: 10.3390/nano12081362

Figure 4.

Figure 4

Initial exposure protocol. For each 4 biological replicates (BR), NR8383 were seeded in 5 wells, in Transwell® inserts. The next day, for each BR, the inserts containing the cells were exposed, by nebulization, back-to-back with 61 µL of whole porcine surfactant at 40 mg/mL, and with 57 µL of TiO2 NP at 30 mg/mL. After exposures, the Transwell® inserts containing the cells were kept at 37 °C for 4 h, and the 5 wells (representing technical replicates, TR) of each BR were pooled for RNA extractions.