Table 2.

Quality and quantity of RNA extracted from NR8383 cells exposed to TiO${}_2$ NP through nebulization following the initial or current protocol. Results in red indicate a poor RNA quality or quantity preventing the use of the sample.

		Initial Protocol (57 $\mathsf{\mu }$L, 30 mg/mL NP)			Current Protocol (114 $\mathsf{\mu }$L, 15 mg/mL NP)
Exposure	Biological Replicate	RNA Quantity ($\mathsf{\mu }$g/$\mathsf{\mu }$L)	OD 260/280	OD 260/230	RNA Quantity ($\mathsf{\mu }$g/$\mathsf{\mu }$L)	OD 260/280	OD 260/230
	1	89.04	1.66	1.84	32.31	2.09	2.09
	2	7.99	2.25	0.46	55.30	2.01	1.80
H${}_2$O	3	121.07	2.12	1.14	69.60	2.13	2.18
	4	206.30	2.04	2.32	16.09	2.08	1.43
	5	/	/	/	48.94	2.18	1.98
	1	3.30	1.86	0.26	3.61	1.83	3.72
	2	96.96	2.03	1.81	61.96	2.14	2.16
TiO${}_2$ NP	3	8.01	2.48	51.25	91.57	1.99	2.05
	4	134.43	2.08	1.55	58.93	2.10	2.02
	5	/	/	/	66.30	2.06	2.17
Mean		83.61	2.07	1.33	50.46	2.06	2.16
SD		73.27	0.24	0.70	26.34	0.10	0.59