Skip to main content
. 2022 Apr 18;12(4):598. doi: 10.3390/biom12040598

Figure 3.

Figure 3

Screening for kinases responsible for Cla4p-S351 and S445. (A–D) Samples were run on 50 µM Phos-tag gels and detected by anti-GFP immunoblotting. Arrows indicate phosphorylated species. (A) The Cla4p truncation (MR6289) was expressed in indicated mutant cells (MY10273, 11198, 13003, 13004, 13005, 13006, 13007, 13010, 13011, 13012, and 13013) under the GAL1 promoter for 90 min after α-factor arrest for 2 h. (B) The indicated Cla4p truncation (MR6289 and 6290) was expressed in SLT2 (MY11198) and slt2Δ (MY13011) under the GAL1 promoter for 90 min during mitosis or after α-factor arrest for 2 h. (C) Actively growing cdc28-as1 (MY11198) was treated with DMSO or the inhibitor, 1-NM-PP1, after which truncated proteins (MR6289 and 6290) were induced by the GAL1 promoter for 90 min. (D) The Cla4p truncation (MR6291) was expressed in indicated mutant cells (MY11198, 13018, 13019, 13020, 13021, 13022, 13025, 13026, 13027 and 13028) under the GAL1 promoter for 90 min after α-factor arrest for 2 h.