Table 2.

Results from larval administration of MeIQx doses.

Doses	No. of Wings (N)	Small Single Spots (1–2 Cells) (m = 2)			Large Single Spots (>2 Cells) (m = 5)			Twin Spots (m = 5)			Total mwh Spots a (m = 2)			Total Spots (m = 2)			Frequency of Clone Formation per 105 Cells b	Recombination c (%)
mwh/flr3 wings		No	Fr	D	No	Fr	D	No	Fr	D	No	Fr	D	No	Fr	D
% 0.5 DMSO	80	23	0.29	-	3	0.04	i	0	0.00	i	26	0.33	i	26	0.33	i	1.33
4.69 µM	80	40	0.43	+	5	0.05	i	1	0.01	i	46	0.50	+	46	0.58	+	2.04	40
9.38 µM	80	42	0.53	+	6	0.08	i	0	0.00	i	48	0.60	+	48	0.60	+	2.46	56,67
23.45 µM	80	41	0.39	+	4	0.04	i	1	0.01	i	46	0.50	+	46	0.58	+	2.04	32
mwh/TM3 wings
% 0.5 DMSO	80	19	0.24	-	2	0.03	-				21	0.26	-	21	0.26	-	1.08
4.69 µM	80	23	0.29	i	1	0.01	i				24	0.30	i	24	0.30	i	1.23
9.38 µM	80	20	0.25	i	1	0.01	i				21	0.26	i	21	0.26	-	1.08
23.45 µM	80	26	0.33	i	1	0.01	i				27	0.34	i	27	0.34	i	1.38

No: number, Fr: frequency, D: statistical diagnosis according to Frei and Wurgler [20], +: positive, -: negative, i: inconclusive, m: multiplication factor, probability levels α = β = 0.05. ^a Considering mwh clones from single and twin spots. ^b Frequency of clone formation: mwh clones/wings/24,400 cells (without size correction) [23]. ^c Recombination (%): [(frequency of mwh clones from mwh/flr³ wings-frequency of mwh clones from mwh/TM3 wings)/frequency of mwh clones from mwh/flr³ wings] × 100 [24].