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. 2022 Apr 13;11(4):518. doi: 10.3390/antibiotics11040518

Figure 2.

Figure 2

Figure 2

Effect of EGCG on membrane permeability. V. cholerae N16961 was treated with EGCG at concentrations of 0.25× MIC to 4× MIC for 2 h at 37 °C. Intracellular leakage of nucleotides (a) and proteins (b) were measured, and 0.1% Triton X-100 (TX) was used as a positive control. The outer membrane disruption and membrane potential dissipation were investigated in terms of the Relative Fluorescence Intensity (RFI) percentages of NPN (c) and Rh123 (d). Significant differences compared to untreated controls are indicated by asterisks: * p < 0.05, ** p < 0.01, *** p < 0.001.