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Establishment of RPA-CRISPR/Cas12a system. (a) Relatively conserved sequence of mecA gene of MRSA shows target and crRNA sequences on the polymerase coding region. (b) Screening for CrRNAs. Real-time fluorescence curves based on PCR-CRISPR/Cas12a on different 3 CrRNAs to detect efficiency. NTC: no template control. (c) Screening of RPA primers. Fluorescence values based on RPA-CRISPR detection at different RPA primers. Gray: Postive; Black: Negative.
