Figure 4.

Determination of the sensitivity of RPA-CRISPR/Cas12a assays. Different concentrations of MRSA Plasmids (10${}^1$ to 10${}^6$ copies per reaction) were used test. (a) Real-time fluorescence RPA-CRISPR/Cas12a detection of different MRSA dilution. NTC: no template control. (b) Endpoint fluorescence/visual detection to MRSA plasmid dilution. (c) LFS-based RPA-CRISPR/Cas12a detection assay of MRSA plasmid dilution. Except for the blurred sample 10 copies/reaction test lines, all samples had clear test lines. (d) qPCR amplification curves and standard curves for MRSA plasmid dilution.