Relative expression levels of P5CS (A), DHN5 (B), WZY2 (C), DREB1 (D), and WRKY2 (E) in wheat seedlings of six Croatian wheat varieties (Silvija, Rujana, Bubnjar, Fifi, Anđelka, and Pepeljuga) under 10 and 20% PEG treatment. Expression data are presented as means of three independent biological replicates, and the error bars indicate standard deviations. Differences among treatments in each variety separately were assessed by a one-way analysis of variance (ANOVA), followed by the Dunnett post hoc test. The Dunnett test was performed at a significance level of 5, 1 and 0.1% (* p < 0.05, ** p < 0.01, *** p < 0.001).