Table 1.
Primary Antibody |
Clone | Dilution | Source | Visualisation System |
---|---|---|---|---|
Iba-1 | Polyclonal rabbit | 1:200 | Wako Pure Chemical Industries, Ltd., Osaka, Japan | Impress HRP Universal Antibody (Anti-Mouse/Rabbit IgG) a |
CD3 | Polyclonal rabbit anti-human | 1:50 | Dako, Glostrup, Denmark | Impress HRP Universal Antibody (Anti-Mouse/Rabbit IgG) a |
CD79a | Monoclonal mouse anti-human HM57 | 1:100 | Bio-Rad Laboratories Inc., Hercules, CA, USA | EnVision+ System-HRP, Mouse (DAB) b |
CD20 | Monoclonal rabbit anti-human SP32 | 1:100 | Abcam, Cambridge, UK | Impress HRP Universal Antibody (Anti-Mouse/Rabbit IgG) a |
Vimentin | Monoclonal mouse anti-bovine VIM 3B4 | 1:100 | Dako, Glostrup, Denmark | EnVision+ System-HRP, Mouse (DAB) b |
Monoclonal mouse anti-porcine V9 | 1:100 | |||
Desmin | Monoclonal mouse anti-human D33 | 1:50 | Dako, Glostrup, Denmark | EnVision+ System-HRP, Mouse (DAB) b |
α-SMA | Monoclonal mouse anti-human 1A4 | 1:50 | Dako, Glostrup, Denmark | EnVision+ System-HRP, Mouse (DAB) b |
a Vector Laboratories Inc., Burlingame, CA, USA. b Dako, Glostrup, Denmark.