Table 3.
Amino acid (aa) substitutions in neutralizing epitopes of VP7 (7-1a, 7-1b, and 7-2) observed in Italian RV strains: (a) G1P[8], (b) G2P[4], (c) G3P[8], (d) and G4P[8], compared to vaccine strains (Rotarix® and/or RotaTeq®).
| (a) G1P[8] | Epitopes | |||||
| 7-1a | 7-1b | 7-2 | ||||
|
Strain/sub-lineage
(No. of sequences analyzed) |
Positions | |||||
| 94 | 212 | 97 | 123 | 147 | 217 | |
| Rotarix® (A41CB052A /1988/G1P1A[8]) | N | V | E | S | N | M |
| RotaTeq® (WI79-9/1992/G1P7[5]) | N | V | D | S | S | M |
| G1P[8] sub-lineage Ic (54) | S | T | E | N | N | T |
| G1P[8] sub-lineage IIa (6) | N | V | E | S/N | N | M |
| (b) G2P[4] | Epitopes | |||||
| 7-1a | 7-1b | |||||
|
Strain/sub-lineage
(No. of sequences analyzed) |
Positions | |||||
| 87 | 96 | 213 | ||||
| RotaTeq® (SC2-9/1992/G2P7[5]) | A | D | S | |||
| G2P[4] sub-lineage IVa-1 (18) | T | N/S | D | |||
| G2P[4] sub-lineage IVa-3 (18) | T | N | D | |||
| (c) G3P[8] | Epitopes | |||||
| 7-1a | 7-1b | |||||
|
Strain/sub-lineage
(No. of sequences analyzed) |
Positions | |||||
| 87 | 212 | 213 | 238 | 242 | ||
| RotaTeq® (WI78-8/1992/G3P75) | T | A | N | K | D | |
| G3P[8] human (24) | T/I | T | N | N | N | |
| G3P[8] equine-like (39) | S | T/A | T | D | A | |
| (d) G4P[8] | Epitopes | |||||
| 7-1a | 7-1b | 7-2 | ||||
|
Strain/sub-lineage
(No. of sequences analyzed) |
Positions | |||||
| 130 | 211 | 143 | 145 | |||
| RotaTeq® (BrB-9/1996/G4Ia) | D | D | R | A | ||
| G4P[8] sub-lineage Ic (14) | E | N | K | T | ||
The letters represent the peculiar amino acid substitutions of neutralization epitopes (7-1a, 7-1b and 7-2) of VP7 compared with homologous sequences of the Rotarix® and RotaTeq® vaccine strains, were showed in Table 3.