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Quality control of purified hESAM-specific Nbs. (A) Coomassie Blue-stained SDS-PAGE of purified Nbs confirming the presence of a single protein band at standard Nb molecular weights; 5 µg of purified Nb eluates were loaded. (B) Western blot analysis using an anti-His IgG antibody to verify the identity of the Nbs; 5 µg of purified Nb eluates were loaded on the SDS-PAGE.
