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. 2022 Apr 15;23(8):4369. doi: 10.3390/ijms23084369

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Whole-mount stainings of entire human skin biopsies using a directly-labeled hESAM-specific Nb. Whole-mount immunofluorescence stained human skin punch biopsies (5 × 5 mm) stained with the ‘2ES41’ Nb clone (white) and optically sectioned using a light sheet microscope. Shown are representative projections of 3D-rendered computer reconstructions. Overview images of three different skin biopsies are shown in the top panels (A,C,E), whereas the bottom panels (B,D,F) depict images with higher magnification of the framed areas. Scale bars = 50 µm.