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. 2022 Apr 13;11(4):771. doi: 10.3390/antiox11040771

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Characterization of Macrophthalmus japonicus p53 gene. (A) ClustalW multiple-sequence alignment of the deduced Mjp53 gene sequence with homologous p53 genes of various crabs. Shaded marks in black indicated completely conserved residues in all species. Dimerization site (polypeptide-binding site) and zinc-binding site (ion-binding site) are indicated by black asterisk mark and blue asterisk mark, respectively. DNA-binding site (nucleic-acid-binding site) is presented as a red rectangular box. (B) Phylogenetic circle tree of Mjp53 gene with other p53s. Neighbor-joining analysis showed a circle tree for phylogenetic relationships in p53 amino acid sequences using the MEGA v.4.0 software. Bootstrap values represent 1000 replicates.