Skip to main content
. 2022 Apr 7;23(8):4081. doi: 10.3390/ijms23084081

Figure 7.

Figure 7

Histochemical detection of GUS expression in rice seeds imbibed in a CNT-plasmid DNA solution. Sterilized rice seeds were soaked overnight in water to initiate germination, then with a osmotic solution of 0.6 mannitol for two hours, followed by a 5-day exposure to a CNT-pDNA solution at 1:3 ratio with a 35S::GUSPlus vector. GUSPlus enzymatic activity was visualized by histochemical assay using standard procedures [32]. (A) Imbibed rice seed in MES delivery buffer (negative control). (B–E) Detection of GUS expression in germinating rice tissues after 5 days in the CNT-pDNA mixture solution.