Figure 4.

Association of SRPX with TMZ resistance in glioblastomas. (A) The mRNA expression levels of SRPX at increasing concentrations of TMZ, and (B) at different time intervals. Relative expression analysis was performed using the ΔΔCq method and gene expression was normalized to hB-actin. Error bars indicate means ± SEM and p-values indicate significance compared to the DMSO treatment (Bonferroni or Dunnett multiple comparisons test). (C) Cell viability. 72 h after transfection, cells were stained with trypan blue and then counted by using a Neubauer cell counting chamber. (D) Colony formation assay showing U251-MG-P colony survival after being exposed to TMZ. Error bars indicate means ± SEM and p-values indicate significance compared to the cells treated with control siRNA (Bonferroni or Dunnett multiple comparisons test).