FIGURE 2.

High Kindlin‐1 expression is associated with the epidermal growth factor receptor (EGFR) pathway in breast cancer cell lines and tumours. (A) Correlation between EGFR and Kindlin‐1 mRNA expression levels in 976 breast tumours from The Cancer Genome Atlas (TCGA) (Spearman's rank correlation test). (B) Gene set enrichment analysis (GSEA) plot showing the enrichment of the EGFR pathway in breast tumours from TCGA with high mRNA expression of Kindlin‐1 (n = 72/976). C. Kindlin‐1 and EGFR immunohistochemical staining in breast tumours from three different patients with luminal A (Lum A) or triple‐negative breast cancers (TNBCs). These tumours are representative cases of a series of 62 carcinomas from patients treated at the Curie Hospital. (D) Scatter plot showing Kindlin‐1 protein expression in the same series as in (D). Cases were categorised as low versus high EGFR protein expression. Each point represents the H‐score from a single tissue sample ranging from total absence of Kindlin‐1 in the epithelial compartment (H‐score 0) to very strong Kindlin‐1 staining (H‐score 300). (E) Kaplan–Meier curve showing metastasis‐free survival of TNBC patients with respect to EGFR and Kindlin‐1 expression. Patients were divided into three categories: low expression of both EGFR and Kindlin‐1 (blue line); single overexpression of EGFR or Kindlin‐1 (green line); concomitant overexpression of EGFR and Kindlin‐1 (red line) (log‐rank test)