Table 2.
Positivity in a nested PCR species-specific for Aelurostrongylus abstrusus of different fecal aliquots and pharyngeal swab collected from cats of the present study. The different combinations of co-infections are detailed.
| Copromicroscopy n/tot (%) |
PCR Feces n/tot (%) |
PCR Flotation n/tot (%) |
PCR Baermann n/tot (%) |
PCR Pharyngeal Swab n/tot (%) |
||
|---|---|---|---|---|---|---|
| Italy | ||||||
| Group A | Aelurostrongylus abstrusus | 16/25 (64) | 10/16 (62.5) | 10/16 (62.5) | 14/16 (87.5) * | 14/16 (87.5) |
| Aelurostrongylus abstrusus + Toxocara cati | 7/25 (28) | 4/7 (57.1) | 6/7 (85.7) | 6/7 (85.7) * | 6/7 (85.7) | |
| Aelurostrongylus abstrusus + Toxocara cati + Toxascaris leonina + Capillaria aerophila | 1/25 (4) | 1/1 (100) | 1/1 (100) | 0/1 (0) | 1/1 (100) | |
| Aelurostrongylus abstrusus + Ancylostomatidae + Capillaria aerophila | 1/25 (4) | 1/1 (100) | 0/1 (0) | 1/1 (100) * | 0/1 (0) | |
| Group B | Toxocara cati | 8/15 (53.3) | 0/8 (0) | 1/8 (12.5) | 2/8 (25) ** | 0/8 (0) |
| Ancylostomatidae | 6/15 (40) | 0/6 (0) | 0/6 (0) | 0/6 (0) | 0/6 (0) | |
| Capillaria aerophila | 1/15 (6.7) | 0/1 (0) | 0/1 (0) | 0/1 (0) | 0/1 (0) | |
| Group C | Negative at copromicroscopy | - | 0/20 (0) | 0/20 (0) | 0/20 (0) | 2/20 (10) |
| Greece | ||||||
| Group A | Aelurostrongylus abstrusus | 16/25 (64) | 16/16 (100) | 13/16 (81.3) | 13/16 (81.3) * | NA |
| Aelurostrongylus abstrusus + Troglostrongylus brevior | 6/25 (24) | 5/6 (83.3) | 4/6 (66.7) | 6/6 (100) * | NA | |
| Aelurostrongylus abstrusus + Troglostrongylus brevior + Toxocara cati + Capillaria aerophila | 1/25 (4) | 1/1 (100) | 0/1 (0) | 1/1 (100) * | NA | |
| Aelurostrongylus abstrusus + Ancylostomatidae | 2/25 (8) | 0/2 (0) | 0/2 (0) | 1/2 (50) | NA | |
| Group B | Toxocara cati | 9/10 (90) | 1/9 (11.1) | 1/9 (11.1) | 2/9 (22.2) ** | NA |
| Ancylostomatidae | 1/10 (10) | 0/1 (0) | 0/1 (0) | 0/1 (0) | NA | |
| Group C | Negative at copromicroscopy | - | 0/5 (0) | 0/5 (0) | 0/5 (0) | NA |
| Total | ||||||
| Group A | Aelurostrongylus abstrusus | 32/50 (70) | 26/32 (81.3) | 23/32 (71.9) | 27/32 (84.4) | 14/16 (87.5) |
| Aelurostrongylus abstrusus + Troglostrongylus brevior | 6/50 (12) | 5/6 (83.3) | 4/6 (66.7) | 6/6 (66.7) | NA | |
| Aelurostrongylus abstrusus + Troglostrongylus brevior + Toxocara cati + Capillaria aerophila | 1/50 (2) | 1/1 (100) | 0/1 (0) | 1/1 (100) | NA | |
| Aelurostrongylus abstrusus + Toxocara cati | 7/50 (14) | 4/7 (57.1) | 6/7 (85.7) | 6/7 (85.7) | 6/7 (85.7) | |
| Aelurostrongylus abstrusus + Toxocara cati + Toxascaris leonina + Capillaria aerophila | 1/50 (2) | 1/1 (100) | 1/1 (100) | 0/1 (0) | 1/1 (100) | |
| Aelurostrongylus abstrusus + Ancylostomatidae | 2/50 (4) | 0/2 (0) | 0/2 (0) | 1/2 (50) | NA | |
| Aelurostrongylus abstrusus + Ancylostomatidae + Capillaria aerophila | 1/50 (2) | 1/1 (100) | 0/1 (0) | 1/1 (100) | 0/1 (0) | |
| Group B | Toxocara cati | 17/25 (68) | 1/17 (5.9) | 2/17 (11.8) | 4/17 (23.5) | 0/17 (0) |
| Ancylostomatidae | 7/25 (28) | 0/7 (0) | 0/7 (0) | 0/7 (0) | 0/7 (0) | |
| Capillaria aerophila | 1/25 (4) | 0/1 (0) | 0/1 (0) | 0/1 (0) | 0/1 (0) | |
| Group C | Negative at copromicroscopy | NA | 0/25 (0) | 0/25 (0) | 0/25 (0) | 2/20 (10) |
* One amplicon subjected to sequencing; ** amplicons subjected to sequencing; n = number of positive animals; tot = number of examined animals; NA = Not Applicable, pharyngeal swabs were collected only for cats enrolled in Italy.