Table 3.
Overall positivity in a nested PCR species-specific for Aelurostrongylus abstrusus of different fecal aliquots and pharyngeal swab collected from cats of the present study.
| PCR Feces n/tot (%; 95% CI) |
PCR Flotation n/tot (%; 95% CI) |
PCR Baermann n/tot (%; 95% CI) |
PCR Pharyngeal Swab n/tot (%; 95% CI) |
Total PCR n/tot (%; 95% CI) |
|
|---|---|---|---|---|---|
| Italy | |||||
| Group A | 16/25 (64; 42.5–82) | 15/25 (60; 38.7–78.9) | 21/25 (84; 63.9–95.5) | 21/25 (84; 63.9–95.5) | 24/25 (96; 79.7–99.9) |
| Group B | 0/15 (0) | 1/15 (6.7; 0.2–32) | 2/15 (13.3; 1.7–40.5) | 0/15 (0) | 2/15 (13.3; 1.7–40.5) |
| Group C | 0/20 (0) | 0/20 (0) | 0/20 (0) | 2/20 (10; 1.2–31.7) | 2/20 (10; 1.2–31.7) |
| Greece | |||||
| Group A | 22/25 (88; 68.8–97.5) | 18/25 (72; 50.6–87.9) | 20/25 (80; 59.3–93.2) | NA | 24/25 (96; 79.7–99.9) |
| Group B | 1/10 (10; 0.3–44.5) | 1/10 (10; 0.3–44.5) | 2/10 (20;2.5–55.6) | NA | 2/10 (20;2.5–55.6) |
| Group C | 0/5 (0) | 0/5 (0) | 0/5 (0) | NA | 0/5 (0) |
| Total | |||||
| Group A | 38/50 (76; 61.8–86.94) | 35/50 (70; 55.4–82.1) | 41/50 (82; 68.6–91.4) | 21/25 (84; 63.9–95.5) | 48/50 (96; 86.3–99.5) |
| Group B | 1/25 (4; 0.1–20.4) | 2/25 (8; 1–26) | 4/25 (16; 4.5–36.1) | 0/15 (0) | 4/25 (16; 4.5–36.1) |
| Group C | 0/25 (0) | 0/25 (0) | 0/25 (0) | 2/20 (10; 1.2–31.7) | 2/25 (8; 1–26) |
n = number of positive animals; tot = number of examined animals; 95% CI = 95% Confidence Interval; NA = Not Applicable, pharyngeal swabs were collected only for cats enrolled in Italy.