Figure 1.
MT-ATP6 as a model substrate for the study of co-translational quality control. (A) Immunoblotting of whole-cell lysates from human fibroblasts treated with the indicated siRNAs. sc, scrambled control. *Non-specific band detected with AFG3L2 antibody. (B) A representative image of 35S-methionine/cysteine metabolic labelling of mitochondrial protein synthesis in human fibroblasts treated with the indicated siRNAs. Cells were pulse-labelled for 30 min, followed by 60 and 180 min cold chase. (C) Quantification of the metabolic labelling from five independent experiments with the mean ± SD.