| Antibodies |
|
|
| Rabbit polyclonal anti-CBX2 |
Abcam |
Cat#80044; RRID: AB_2049270 |
| Rabbit polyclonal anti-CBX2 |
Bethyl |
Cat#A302–524A; RRID: AB_1998943 |
| Rabbit monoclonal anti-LIN28B |
Cell Signaling Technology |
Cat#11965; RRID: AB_2750978 |
| Rabbit monoclonal anti-PU.1 |
Cell Signaling Technology |
Cat#2258; RRID: AB_2186909 |
| Rabbit monoclonal anti-Ubiquityl-Histone H2A (Lys119) |
Cell Signaling Technology |
Cat#8240; RRID: AB_10891618 |
| Rabbit monoclonal anti-β-Actin |
Cell Signaling Technology |
Cat#4970; RRID: AB_2223172 |
| B220-Pacific blue (RA3–6B2) |
BioLegend |
Cat#103227; RRID: AB_492876 |
| B220-PE-Cy7 (RA3–6B2) |
BioLegend |
Cat#103222; RRID: AB_313005 |
| CD117-APC-Cy7 (2B8) |
BioLegend |
Cat#105826; RRID: AB_1626278 |
| CD150-APC (TC15–12F12.2) |
BioLegend |
Cat#115910; RRID: AB_493460 |
| CD16/32-PerCP-Cy5.5 (93) |
BioLegend |
Cat#101324; RRID: AB_1877267 |
| CD19-PE (eBio1D3) |
eBioscience |
Cat#12–0193-85; RRID: AB_657662 |
| CD3-Pacific blue (17A2) |
BioLegend |
Cat#100214; RRID: AB_493645 |
| CD3-PE (17A2) |
BioLegend |
Cat#100206; RRID: AB_312663 |
| CD34-FITC (RAM34) |
eBioscience |
Cat#11–0341-85; RRID: AB_465022 |
| CD45.1-APC-Cy7 (A20) |
BioLegend |
Cat#110716; RRID: AB_313505 |
| CD45.2-FITC (104) |
BioLegend |
Cat#109806; RRID: AB_313443 |
| CD48-FITC (HM48–1) |
BioLegend |
Cat#103404; RRID: AB_313019 |
| CD5-APC-Cy7 (53–7.3) |
BioLegend |
Cat#100650; RRID: AB_2876396 |
| Flk2-PE (A2F10) |
BioLegend |
Cat#135306; RRID: AB_1877217 |
| Gr-1-Pacific blue (RB6–8C5) |
BioLegend |
Cat#108430; RRID: AB_893556 |
| Mac-1-PE-Cy5 (M1/70) |
BioLegend |
Cat#101210; RRID: AB_312793 |
| Sca-1-PE- Cy7 (D7) |
BioLegend |
Cat#108114; RRID: AB_493596 |
| Ter119-APC-Cy7 (TER-119) |
BioLegend |
Cat#116223; RRID: AB_2137788 |
| Ter119-Pacific blue (TER-119) |
BioLegend |
Cat#116232; RRID: AB_2251160 |
|
| Chemicals, peptides, and recombinant proteins |
|
|
| APC-annexin V |
BioLegend |
Cat#640919 |
| Dexamethasone |
Sigma-Aldrich |
Cat#D4902 |
| Doxycycline |
Sigma-Aldrich |
Cat#D9891 |
| Geneticin™ Selective Antibiotic |
Thermo Fisher Scientific |
Cat# 10131035 |
| Human EPO |
PeproTech |
Cat#500-P318 |
| Human SCF |
R&D |
Cat#255-SC |
| Power SYBR™ Green PCR Master Mix |
Thermo Fisher Scientific |
Cat#4367659 |
| SuperScript™ II Reverse Transcriptase |
Thermo Fisher Scientific |
Cat#18064 |
| TRIzol™ Reagent |
Thermo Fisher Scientific |
Cat#15596026 |
|
| Critical commercial assays |
|
|
| CD117 MicroBeads, mouse |
Miltenyi Biotec |
Cat#130–091-224 |
| CUTANA™ ChIC/CUT&RUN Kit |
EpiCypher |
Cat#14–1048 |
| miScript II RT Kit |
QIAGEN |
Cat#218161 |
| Nano-Glo® Dual-Luciferase® Reporter Assay System |
Promega |
Cat#N1610 |
| SYTOX™ Blue Dead Cell Stain |
Life Technologies |
Cat#S34857 |
| SYTOX™ Red Dead Cell Stain |
Life Technologies |
Cat#S34859 |
|
| Deposited data |
|
|
|
| Raw data |
This paper |
phs002507 |
| Raw and analyzed data |
This paper |
GSE179160 |
|
| Experimental models: Cell lines |
|
|
|
| Human: HEK-293T |
ATCC |
RRID:CVCL_0045 |
| Human: HUDEP-1 |
RIKEN |
RRID:CVCL_VI05 |
| Human: HUDEP-2 |
RIKEN |
RRID:CVCL_VI06 |
| Human: K562 |
ATCC |
RRID:CVCL_0004 |
|
| Experimental models: Organisms/strains |
|
|
|
| Mouse: C57BL/6J |
The Jackson Laboratory |
JAX:000664 |
| Mouse: B6.SJL-Ptprca Pepcb/BoyJ |
The Jackson Laboratory |
JAX: 002014 |
| Mouse: B6Ei.129P2(C)-Cbx2tm1Cim/EiJ |
The Jackson Laboratory |
JAX: 006002 |
| Mouse: iLet7 |
Rowe et al. (2016a)
|
N/A |
| Mouse: iLIN28B |
Rowe et al. (2016b)
|
N/A |
| Zebrafish: Tg(rag2:GFP) |
Traver et al. (2003)
|
ZFIN: ZDB-TGCONSTRCT-070117–56 |
| Zebrafish: Tg(mpo:GFP) |
Renshaw et al. (2006)
|
ZFIN: ZDB-ALT-070118–2 |
|
| Oligonucleotides |
|
|
|
| Splice-blocking morpholino oligonucleotides: cbx2: TAGTTTCCTGAGAGAGGAACACAAA |
This paper |
N/A |
| Primer for human CBX2: Forward: GACAGAACCCGTCAGTGTCC |
This paper |
N/A |
| Primer for human CBX2: Reverse: GGCTTCAGTAATGCCTCAGGT |
This paper |
N/A |
| Primer for human GAPDH: Forward: GTCTCCTCTGACTTCAACAGCG |
This paper |
N/A |
| Primer for human GAPDH: Reverse: ACCACCCTGTTGCTGTAGCCAA |
This paper |
N/A |
| Primer for human GFI1: Forward: GAGCCTGGAGCAGCACAAAG Reverse: GTGGATGACCTCTTGAAGCTCTTC |
This paper |
N/A |
| Primer for human GFI1: Reverse: GTGGATGACCTCTTGAAGCTCTTC |
This paper |
N/A |
| Primer for human SPI1: Forward: GACACGGATCTATACCAACGCC |
This paper |
N/A |
| Primer for human SPI1: Reverse: CCGTGAAGTTGTTCTCGGCGAA |
This paper |
N/A |
| Primer for human ERG: Forward: GGACAGACTTCCAAGATGAGCC Reverse: CCACACTGCATTCATCAGGAGAG |
This paper |
N/A |
| Primer for human ERG: Reverse: CCACACTGCATTCATCAGGAGAG |
This paper |
N/A |
| Primer for mouse b-Actin: Forward: ACGAGGCCCAGAGCAAGAGAGG Reverse: ACGCACCGATCCACACAGAGTA |
This paper |
N/A |
| Primer for mouse b-Actin: Reverse: ACGCACCGATCCACACAGAGTA |
This paper |
N/A |
| Primer for mouse Erg: Forward: GAGTGGGCGGTGAAAGAATA Reverse: TCAACGTCATCGGAAGTCAG |
This paper |
N/A |
| Primer for mouse Erg: Forward: Reverse: TCAACGTCATCGGAAGTCAG |
This paper |
N/A |
|
| Recombinant DNA |
|
|
| pCW57.1 |
Gift from David Root |
Addgene plasmid # 41,393 |
| pGL4.50 |
Promega |
Cat#E1310 |
| pNL3.1 |
Promega |
Cat#N1031 |
| pNL3.1-Erg |
This paper |
N/A |
| pMSCV-neo let-7g |
Kumar et al. (2007)
|
Addgene plasmid #14784 |
| pMSCV-puro let-7 sponge |
Kumar et al. (2007)
|
Addgene plasmid # 29,766 |
|
| Software and algorithms |
|
|
| CellNet algorithm |
Cahan et al. (2014)
|
http://cellnet.hms.harvard.edu
|
| CUT&RUNTools |
Zhu et al. (2019)
|
http://broadinstitute.github.io/picard/
|
| FlowJo |
Becton, Dickinson and Company |
https://www.flowjo.com/
|
| HOMER |
Feng et al. (2012)
|
http://liulab.dfci.harvard.edu/MACS
|
| ImageJ |
NIH |
https://imagej.nih.gov/ij/
|
| Targetscan algorithm |
Agarwal et al. (2015)
|
http://www.targetscan.org/vert_80/
|
