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. 2022 Apr 6;11(4):723. doi: 10.3390/antiox11040723

Figure 3.

Figure 3

Effect of SIRT3 overexpression on SOD2 activation and mitochondrial oxidative stress in control and hypertrophied neonatal rat cardiomyocytes (NCMs). Control (PBS) or Iso-treated NCM were transfected with an empty vector (PCMV6) (gray box) or SIRT3 cDNA (3 µg, dark blue box) for 72 h. Quantification by Western blot of SIRT3 and SIRT1 expression (a) and SOD2acK68/SOD2 ratio and SOD2 expression (b) in Iso-treated NCM transfected with control or with SIRT3. Data were normalized on actin. (c) Mitochondrial superoxide anions production was detected after MitoSOX (red) and nuclei (blue) staining and quantified from 3 independent experiments and at least 168 cells. (d) Cell area was quantified from 3 independent experiments and at least 51 cells after alpha-actinin (red) and nucleus (blue) staining. Scale bar = 20 µm. Only significant p values are indicated from 3 independent experiments. Images were selected to represent the mean values of each condition.