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. 2022 Apr 13;27(8):2499. doi: 10.3390/molecules27082499

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(A,B) Gel retardation assay (GRA) in guinea pig cytosol for the investigation of the behavior of CS, CA, 7MER, DMA, BA and R4 versus AhR in the presence and absence of 20 nM of TCDD. Time of incubation 1.5 h. Results are expressed as means ± SD. (7MER = 7-O-methyl-epi-rosmanol, DMA = 4′,7-O-dimethylapigenin, CS = carnosol, CA = carnosic acid, BA = betulinic acid, R4 = total methanolic extract). The bar means followed by the same letter (as superscript) are not statistically different at p ≤ 0.01.

(A,B) Gel retardation assay (GRA) in guinea pig cytosol for the investigation of the behavior of CS, CA, 7MER, DMA, BA and R4 versus AhR in the presence and absence of 20 nM of TCDD. Time of incubation 1.5 h. Results are expressed as means ± SD. (7MER = 7-O-methyl-epi-rosmanol, DMA = 4′,7-O-dimethylapigenin, CS = carnosol, CA = carnosic acid, BA = betulinic acid, R4 = total methanolic extract). The bar means followed by the same letter (as superscript) are not statistically different at p ≤ 0.01.