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. 2022 Mar 24;10(4):504. doi: 10.3390/vaccines10040504

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The graphs represent IL-5 and IFN-gamma secreted by spleen cells after vaccinating murine groups with the formulated vaccines. Graphs (A,B) show IL-5 and IFN-gamma secreted in blood collected on sacrificing day (post the second immunizing dose) and analyzed by ELIspot. Samples tested antigens used were cell culture medium (M) as negative control and 1 µg/mL of recombinant S1 spike protein, S1 receptor-binding motif antigen (S1 RBM) and 1 µg/mL nucleoprotein (NC) with cells from the respective study group. Data are driven from five individuals, p value < 0.05.