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Validation of differentially expressed miRNAs. Expression levels of miR-195a-5p, miR-497a-5p, miR-99a-5p, and miR-199a-3p on miRNAs isolated from colonic IECs. miRNAs expression levels were quantified using qRT-PCR and the relative expression was normalized to the expression of endogenous control miR-186-5p. All tested miRNAs showed a significant change in expression, confirming microarray data. * p < 0.05; ** p < 0.01.
