Figure 3.

miR-195a-5p targets CLDN2. (A) Sequence alignment of the miR-195a-5p base-pairing sites in the 3’-UTR of Cldn2 mRNA showing that the regions complementary to miR-195a-5p are highly conserved among mouse, human, and chimp. The “seed” sequences of miR-195a-5p complementary to Cldn2 are shown in white. (B) Cldn2 mRNA expression levels evaluated in the same set of RNA samples used in the microarray validation. Cldn2 levels were significantly higher in Winnie mice compared to wild-type mice. Cldn2 expression levels were normalized on the housekeeping gene Gapdh. The histograms represent the mean ± SEM. * p = 0.03. (C) Linear correlation between the expression of Cldn2 and the expression of miR-195a-5p. Cldn2 mRNA levels inversely correlated with miR-195a-5p expression levels. Black points represent correlation between mRNA and miRNA expression for each sample (r = −0.64; p = 0.04).