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. 2022 Apr 16;10(4):919. doi: 10.3390/biomedicines10040919

Figure 4.

Figure 4

miR-195-5p regulates Cldn2 mRNA expression. (A) Cldn2 mRNA expression was analyzed by Real-Time PCR after transfection with miR-195-5p mimic in HT-29, Caco2, and T84 human cell lines. The increased intracellular amount of miR-195-5p (at 30 nM and 50 nM concentrations) led to a significant decrease of Cldn2 in all cell lines. (B) Cldn1 mRNA expression was analyzed by Real-Time PCR after transfection with miR-195-5p mimic in HT-29, Caco2, and T84 human cell lines. The levels of Cldn1 remained similar to mock-control in all cell lines. Expression data were normalized to the housekeeping gene Gapdh. Data are representative of four independent experiments (mean ± SEM). * p < 0.01, ** p < 0.001, *** p < 0.0001.