Figure 7.

Summary of competition assays using alanine-substituted NW peptide. (A) Carton diagram of the NW peptide (side view). Amino acids represented as lines are indicated. (B) Representative histograms showing the inhibitory effect of alanine-substituted peptides on the NW peptide (0.05 μg/mL) binding to monocytes. Four concentrations (1–4) of each peptide competitor were tested. (C) Percentages of inhibition were calculated from the geometric mean fluorescence intensity. Average mean values and SD of three independent experiments are shown. The numbers 1 to 4 correspond to the used peptide concentrations. * p < 0.05, ** p < 0.01, *** p < 0.005, and **** p < 0.0002.