Figure 9.

Competition assays using peptides with the neutral replacement of W or L. (A,B) Representative histograms showing the inhibitory effects of tyrosine—(Y)-, phenylalanine—(F)-, or valine—(V)-substituted peptides on the biotinylated NW peptide binding to monocytes. Experimental conditions are as in Figure 7. The reference NW peptide (WT) was included as a positive control. (C) Percentages of inhibition were calculated from the geometric mean fluorescence intensity. Average mean values and SD of three independent experiments are shown. ** p < 0.01, *** p < 0.001, and **** p < 0.0002.