Overlay of VOC Gamma spike protein mutations identified in the state of Santa Catarina during the COVID-19 pandemic. Residues involved in mutations are presented as spheres, colored from blue to red (low to high mutation frequency). The mutation sites are also indicated in the two-dimensional representation of the spike protein, which is colored according to protein regions. The NTD domain is colored in green and the RBD domain is colored in purple. From the total 778 analyzed sequences of the VOC Gamma variant (5′–3′), red represents a frequency of 93.6–100%, orange a frequency of 88.8%, yellow a frequency of 3.98%, green a frequency of 1.79%, and blue a frequency of less than 1%. Image generated using PyMOL version 2.3.3 [35].