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. 2022 Apr 18;23(8):4469. doi: 10.3390/ijms23084469

Figure 3.

Figure 3

Coinfection of PCV2 and PRV regulates IRFs. (A,B) Expression levels of IRFs. The mRNA levels of IRF3 (A) and IRF7 (B) were evaluated via real-time PCR. (C) Protein levels of IRF7. Western blotting was performed using Rabbit Anti-IRF7 antibody and Anti-β-Actin Antibody as primary antibodies, respectively. HRP-labeled Goat Anti-mouse IgG (H+L) and HRP-labeled Goat Anti-rabbit IgG (H+L) were used as the secondary antibody. β-actin was used as a control. The average expression level of the target protein in each group is shown below each lane. The protein amount of the PK-15 group is set to 1, and the values of other groups are the ratio with the PK-15 group. (D,E) Expression levels of ISGs. The mRNA levels of ISG15 (D) and ISG56 (E) were evaluated via real-time PCR. *, p-value < 0.05; **, p-value < 0.01; ***, p-value < 0.001; ****, p-value < 0.0001. The data are presented as the means ± SD. Unprocessed original images can be found in Supplementary Figure S2.