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. 2022 Apr 18;23(8):4469. doi: 10.3390/ijms23084469

Figure 6.

Figure 6

Coinfection of PCV2 and PRV modulates expressions of host pro-inflammatory factors. (A–D) Expression levels of pro-inflammatory factors. The relative mRNA levels of IL1α (A), TNFα (B), IL6 (C), and IL1β (D) were evaluated using real-time PCR. The data are presented as the means ± SD. *, p-value < 0.05; **, p-value < 0.01; ***, p-value < 0.001; ****, p-value < 0.0001. (E) Protein levels of pro-inflammatory factors. Western blotting was performed using IL1α, IL1B Antibody, IL6, TNFα, and Anti-β-Actin Antibody as primary antibodies, respectively. HRP-labeled Goat Anti-mouse IgG (H+L) and HRP-labeled Goat Anti-rabbit IgG (H+L) were used as the secondary antibody. β-actin was used as a control. The average expression level of the target protein in each group is shown below each lane. The protein amount of the PK-15 group is set to 1, and the values of other groups are the ratio with the PK-15 group. Unprocessed original images can be found in Supplementary Figure S5.