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. 2022 Apr 8;14(8):1878. doi: 10.3390/cancers14081878

Figure 3.

Figure 3

ABCA1 suppression reduced the size of epithelial ovarian cancer (EOC) spheroids. (A) Representative photographs (left panels) and column graphs (right panels) showing the effect of ABCA1 suppression on the size of HEY and 27/87 EOC spheroids at 72 h after seeding into low-adhesion plates and 96 h after transfection. n = 3. p-Values were derived from one-sample t-tests. (B) Changes in the number of cells per spheroid after ABCA1 suppression in HEY and 27/87 cells. Assays performed at the same time as for (A). n = 3. p-Values were derived from one-sample t-tests. (C) Representative photographs (left panels) and column graphs (right panels) showing the effect of ABCA1 suppression on the size of WEHI-CS62 patient-derived, high-grade serous EOC spheroids at 72 h after seeding into low-adhesion plates and 96 hours after transfection. n = 3. p-Values were derived from one-sample t-tests. (D) The effect of ABCA1 overexpression, achieved using T0901317, on WEHI-CS62 spheroid size. n = 3. p-Values were derived from unpaired, two-tailed t-tests. (E) The effect of ABCA1 re-expression, achieved using T0901317, on HEY spheroid size. n = 3. (F) The effect of ABCA1 re-expression, achieved using T0901317, on 27/87 spheroid size. n = 3. All results represent the mean ± SD. p-Values derived from one-way ANOVA with Dunnett’s multiple comparison tests. Scale bars represent 500 µm.