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. 2022 Apr 8;14(8):1878. doi: 10.3390/cancers14081878

Figure 5.

Figure 5

ABCA1 suppression induced cholesterol accumulation in serous ovarian cancer cells which was associated with decreased growth in the EOC cells. (A) Changes in intracellular cholesterol levels following ABCA1 suppression. n = 5 for serous HEY and WEHI-CS62 cells. n = 3 for endometrioid 27/87 cells. (B) Cholesterol depletion using methyl-B-cyclodextrin (MBCD) reversed the effect of ABCA1 suppression on 3D growth characteristics of HEY (upper panels) and WEHI-CS62 (lower panels). Experiments were performed at 144 h in 3D culture or 168 h after transfection for HEY and 72 h in 3D culture or 96 h after transfection for WEHI-CS62. n = 5 for HEY and n = 3 for WEHI-CS62 cells. (C) Changes in the number of cells per spheroid after ABCA1 suppression with or without MBCD treatment performed at same time points as for (B). n = 5 for HEY and n = 3 for WEHI-CS62 cells. All results represent mean ± SD. p-Values for (A) were derived from one-sample t-test, whilst all other p-values were derived from two-way ANOVA with Tukey’s multiple comparison tests. Scale bars represent 500 µm.